ZFIN Image: Fetsko et al., 2022, Fig. 4

Image

Figure Caption

Fig. 4

Fig. 4. Zebrafish kdrl, but not gpr124, mutants, express BBB markers. (A) Representative confocal microscopy images showing Claudin-5 staining. WT, kdrl, and gpr124 larvae were stained with ?-Claudin-5 and ?-Glut1. The plvap:EGFP transgene was used as a blood vessel marker. (B) Quantification was calculated from the fraction of the total blood vessels in the hindbrain labeled with ?-Claudin-5. (C, D) Representative confocal images of ?-Pgp staining at 5 dpf with the same controls as in (A) and quantification strategy as in (B). All images are lateral views (anterior left; dorsal top) of whole-mount stained larvae at 5 dpf. Scale bars are 100 ??m for the merged images (left panels) and 40 ??m for the zoomed images. Data in B and D are presented as means (n ?= ?3) ?� ?SEM (???p ?< ?0.001; ns ?= ?not significant).

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 494, Fetsko, A.R., Sebo, D.J., Taylor, M.R., Brain endothelial cells acquire blood-brain barrier properties in the absence of Vegf-dependent CNS angiogenesis, 46-59, Copyright (2022) with permission from Elsevier. Full text @ Dev. Biol.