FIGURE 1

FIGURE 1

Transient expression of IFT46:GAL4-VP16 in ciliated cells. (A) Schematic representation of the 5′ upstream regulatory region of the IFT46 gene that contains putative two RFX2 transcription factor-binding site sequences (red arrowheads). (B) Schematic view of the Tol2-based construct containing GAL4-VP16 under the control of the 2.4 kb IFT46 promoter. (C) Two-color in situ hybridization of the GAL4-VP16 chimeric transcription factor (red) with odf3b (blue) at 24 hpf. GAL4-VP16 transcripts are expressed in multi-ciliated cells (box region) in the distal segment of the pronephric duct (red arrows). Scale bar: 200 μm (C).