Fig. 6

Fig. 6 Depletion of Upf3a but not of Upf1 obviously alters the DEGs in the leg1a^zju1/zju1 mutant.

a, b DEGs between WT and leg1a^zju1/zju1 (leg1a_mu) at 3 dpf (a) and 5 dpf (b) were identified by analyzing the RNA-seq data, respectively. The heat-map shows the hierarchical clustering of these DEGs in the RNA-seq samples obtained from upf3a^−/− (upf3a_mu) single and upf3a^−/−leg1a^zju1/zju1 (upf3a;leg1a_dm) double mutant embryos at 3 dpf (a) and 5 dpf (b), respectively. c, d DEGs between WT and leg1a^zju1/zju1 (leg1a_mu) at 3 dpf (a) and 5dpf (b) were identified by analyzing the RNA-seq data, respectively. The heat-map shows the hierarchical clustering of these DEGs in the RNA-seq samples obtained from upf1^−/− (upf1_mu) single and upf1^−/−leg1a^zju1/zju1 (upf1;leg1a_dm) double mutant embryos at 3 dpf (c) and 5 dpf (d), respectively. e The qPCR analysis of leg1a and leg1b transcripts in WT and leg1b^zju1/zju1 at 1.5 dpf. The embryos were injected with st-MO, upf1-MO, or upf3a-MO at the one-cell stage.