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Fig. 2
Establishment of transgenic zebrafish lines expressing rare minnow Zps
A: Zp proteins identified in EE of rare minnow by LC-MS/MS. B: Upper: Cartoon showing zebrafish zp3.2 promoter-rare minnow zp-eGFP expression construct; Lower: Gel image showing GFP PCR products amplified from transgenic embryos. Ctr: zp3.2: eGFP constructs. C: Fluorescence image showing GFP signals in F0 transgenic ovaries. White arrows indicate oocytes with strong GFP signals. D: Image showing GFP expression in transgenic eggs from F0 parents. White arrow indicates ovulated egg with GFP signals on EE. E: Representative images showing GFP signals in the gonads of indicated transgenic lines. White arrows indicate GFP-positive gonads. F: IF images showing expression of FLAG-tagged Zp3a-eGFP protein in isolated EE of control and transgenic eggs using FLAG antibodies. Note: punctated signals were observed only on EE of transgenic eggs. Bar: 200 μm.