ZFIN Image: Zhang et al., 2022, Fig. 1

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Figure Caption

Fig. 1

TGF‐β decreases the abundance of glycosphingolipid (GSL)‐glycans

A, B
Average copy numbers of (A) total GSL‐glycans and (B) individual GSL‐glycans per cell in NMuMG cells stimulated with TGF‐β.
C, D
Average copy numbers of (C) total GSL‐glycans and (D) individual GSL‐glycans per cell in A549‐VIM‐RFP cells treated with TGF‐β.
E
GSL biosynthesis scheme. GSLs present in both NMuMG cells, and A549‐VIM‐RFP cells are highlighted by red boxes.
F, G
Immunoblot analysis of ST3GAL5 in (F) NMuMG and (G) A549‐VIM‐RFP cells treated with vehicle control or TGF‐β for the indicated time. Vinculin and GAPDH: loading controls.

Data information: Unless stated otherwise, TGF‐β (2.5 ng/ml) and vehicle control were applied for 48 h. The histograms show the mean ± SD values from three biological replicates (n = 3). *P ≤ 0.05; **P < 0.01 based on unpaired Student's t‐test. GT1b: internal normalization control.

Acknowledgments

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