Fig 11

Fig 11 Def regulates liver development only in part through the p53 pathway.

(A–D) WISH analysis of liver development (A) and histogram showing the statistical data for the ratio of P-H3- (B) or PCNA- (C) positive cells in def^-/- embryos injected with p53-specific morpholino mixes (ATG-MO plus spl-MO) at 3 dpf. Number of embryos used for sectioning and number of sections used for counting P-H3- and PCNA-positive hepatocytes in different genotypes in (B) and (C) was shown in (D). (E) WISH analysis of liver development in the def^-/-p53^M214K double mutant at 3dpf. The fabp10a probe was used for WISH. Liver size is measured by liver area marked by the fabp10a-positive signal. In the quartile boxplot, each dot represents the liver size of an individual embryo. st-MO, standard control morpholino; ATG-MO, morpholino targeting the translation start site (ATG) of p53; spl-MO, morpholino targeting the splicing junction of exon 5 and intron 5 of p53. In (A) and (E), total number of embryos used for each genotype is shown on the top of the graph. Underlying data for (A), (B), (C), and (E) are provided in S1 Data.