ZFIN Image: Guan et al., 2016, Fig 9

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Figure Caption

Fig 9 Simultaneous phosphorylations at S87 and S92 are necessary for the nucleolar localisation of Capn3b

(A) Co-IP analysis of the interaction between Capn3b and Def, Def_S87,92A, or Def_S87,92E. The 293T cells were transfected with HA-capn3b^C120S plasmid alone or in combination with Myc-def, Myc-def_S87,92A, or Myc-def_S87,92E plasmid in three different ratios (HA-capn3b:Myc-def^m ratio: 1:1, 1:3, or 1:5), and total protein was extracted 2 d after transfection. Antibodies against the HA-tag (α-HA) or Myc-tag (α-Myc) were used in western blotting. (B, C) Co-immunostaining of the endogenous Def and Capn3b in the liver of 6.5-dpf zebrafish of different genotypes. The percentage of Capn3b-positive cells in different genotypes in (B) was summarized in (C). Nucleoli are indicated by arrows. Scale bar: 10 μm. Underlying data for C are provided in S1 Data.

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