Fig 6

Fig 6 Simultaneous phosphorylations at S58 and S62 or at S87 and S92 are necessary for Def to promote liver development

(A–C) Histogram showing the rescue rate of liver development in def^-/- embryos injected with Myc-tagged def and def_S50A(A), def_S58A, def_S62A, def_S87A, and def_S92A(B), or def_S58,62A and def_S87,92A mRNA (C). Injected embryos at 3.5 dpf were examined by WISH using the fabp10a probe (numbers of def^-/- embryos examined are shown above the bar), and the sizes of liver were classified into small, medium, and normal three groups. (D, E) WISH analysis of liver (red arrow) and exocrine pancreas (blue arrow) development in different transgenic fishes at 4 dpf using the fabp10a (for liver) and trypsin (for exocrine pancreas) probes simultaneously. (F) Each transgenic fish line was crossed to the reporter line Tg(fabp10a:dsRed; elastase:GFP); background and liver volumes were obtained by 3-D reconstruction of the DsRed signal acquired using a confocal microscope. Total number of embryos used for each genotype was shown above each bar. Data are presented as means with SD. Columns with no common letter are significantly different (p < 0.001, one-way ANOVA with Tukey’s post hoc test). Underlying data for A, B, C, and F are provided in S1 Data.