ZFIN Image: Guan et al., 2016, Fig 4

Image

Figure Caption

Fig 4 Def is phosphorylated at its N-terminus.

(A) Western blot of endogenous Def in protein extracts from 32-hpf embryos treated with (+) or without (-) CIP for 1 h at 37°C. (B) Diagram showing different Def derivatives used in (C, D). (C and D) Western blot to detect Myc-tagged (using anti-Myc antibody) (C) or EGFP-tagged (using anti-EGFP antibody). (D) Def derivatives treated with (+) or without (-) CIP. (E) EGFP-D14 was mixed with EDTA or Na₃VO₄ prior to addition of CIP. An anti-EGFP antibody was used in western blot. Phos-Tag: Phos-Tag gel for western blot (A, C).

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