Fig. 3

Fig. 3 Metastatic uveal melanoma zebrafish model is suitable for drug screening.

A Scheme representing the approach to perform drug screening in the here established metastatic uveal melanoma zebrafish model utilizing spXmm66 cell engraftment at 48 hpf followed by tumor burden analysis 6 dpi. B–D Stereomicroscopic images of representative phenotypes of Tg(fliGFPx casper) zebrafish larvae 6 days post injection with tdTomato-labelled spXmm66 cells and treatment with the indicated drugs at their maximum tolerated dose (>80% survival, Supplementary Fig. 1). Scale bars: 250 µm. E Quantitative analysis of (B–D) to determine the effect of the indicated drug treatment on tumor burden, normalized to DMSO control (normalized tumor burden). Data are mean ± SD. *:p < 0.05; **:p < 0.01; ***:p < 0.001.