Fig. 2

Fig. 2 Spheroid cultures can readily be established from both primary uveal melanoma tumors and patient-derived metastatic uveal melanoma tissues derived from murine xenografts.

A Representative image of the established spheroid cultures. Primary: uveal melanoma patient tumor tissue. Metastatic: murine xenograft material, derived from metastatic UM samples propagated subcutaneously. Scale bars: 250 µm). B Analysis of spXmm26 by H&E as well as melanA staining (marker for the melanocytic origin of the engrafted cells). C Stereomicroscopic images of representative phenotypes of Tg(fliGFPx casper) zebrafish larvae (blood vessels: green) 6 days post injection with CM-DiL-labelled spheroid cells (spUM-LB048 and spUM-LB049, red) derived from primary uveal melanoma tumor tissues. Note, disseminated cancer cells are present up to 6 days post engraftment and settle in both the hematopoietic tissue and/or the liver. Inserts: same fish imaged with bright field microscopy.