FIGURE 2

FIGURE 2

Comparison of the effects of the three Kampo-hozai on low sociability in NPY-KO zebrafish. (A–F) The NPY-KO zebrafish were fed control, NYT, HET, or JTT twice daily for 4 days (3% concentration of NYT, HET, or JTT in the diet). Controls were fish fed feed without Kampo-hozai. Sociability was assessed using a three-Chambers test. (A) Experimental scheme for behavioral evaluation. (B) Test apparatus. (C) Tracking of swimming behavior of fish fed control, NYT, HET, and JTT. (D) Time spent in the fish chamber area by the control and three Kampo-hozai-fed NPY-KO zebrafish. (E) Total distance traveled by the control and three Kampo-hozai-fed NPY-KO zebrafish. (F) Time spent in the empty chamber area by the control and three Kampo-hozai-fed NPY-KO zebrafish. Control n = 11, NYT n = 9, HET n = 6, JTT n = 6. Results are shown as mean ± standard deviation. n.s., not significant. (a,b) Groups with different letters in the same row are significantly different by one-way ANOVA-test with Tukey’s multiple comparison test (p < 0.01).