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Fig. 3.
mApple as a bright red fluorophore for zebrafish transgenesis. (A-D) Comparison of membrane-localized mApple-CAAX with EGFP-CAAX in stable sox17:mAppleCAAX and sox17:EGFPCAAX lines show matching expression in the developing pharyngeal endoderm at 28 hpf. Pharyngeal pouches shown in greater detail with anterior towards the left in max projection (A,C) and single slice views (B,D). (E-G) mApple fusion proteins for different localizations. Transient expression of cytoplasmic mApple (E), nuclear nls-mApple (F) and membrane-bound mApple-CAAX (G). Scale bars: 50 µm.
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