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Fig 4 Result of terminal deoxynulceotidyl transferase nick-end-labeling (TUNEL) staining.
(A) The merged image of signals for fluorescein isothiocyanate (FITC) (apoptotic cells) and 4′,6-diamidino-2-phenylindole (DAPI) (nucleus); red arrows and boxes represent site of apoptosis aggregation; scale bar = 1 mm, magnification = ×40. (B) Number of apoptotic cells; Independent sample t-test; n = 3; *P < 0.05, **P < 0.01, ***P < 0.001.
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