Figure 2

Figure 2

Quality control of prepared intestinal single cell suspension from Tg(lyz:DsRED2);Tg(mpeg1:EGFP). (A) Imaging by automated cell counter, cell under brightfield and fluorescence channels (FL1: 488nm; FL2:558nm). Scale bar: 100μm. The cellular diameter of the prepared intestinal mucosal cell suspension was calculated besides. (B) Cell viability reflected AO/PI staining during cytometric analysis. The stained cell number was counted by automatic cell counter. After AO/PI staining, 75% cell obtained from robbing method (RM) was alive, meanwhile for the blowing method, 80% of all cells (AC) and 90% of genetically fluorescence labeled immune cells, which were the target cells (TC), were alive for flow cytometric analysis. (C) Images of intestinal mucosal cells from Tg(lyz:DsRED2);Tg(mpeg1:EGFP). In single cell suspensions, DsRed labeled lyz⁺ cell and EGFP labeled mpeg1⁺ cells could be clearly observed, together with other unstained cells (shown in the brightfield). ** represented p < 0.01, **** represented p < 0.0001.