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Figure 6
L-Tyr inhibits M. tuberculosis (Mtb) intracellular survival via ROS. Macrophages pretreated with L-Tyr (10 μM) or NAC (5 mM) were infected with Mtb at indicated times and subjected to survival analyses. MPMs and Mtb residents were imaged using fluorescence microscopy. Survival rates were calculated by dividing total intracellular GFP-positive signals by total intracellular mCherry-positive signals (a). Survival ratios were calculated by dividing the results at 24 h to those at 3 h (b). Individual values are shown in a scatter dot plot in (a,b). Student’s t-tests (b) were used for analyses.
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