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Figure 1
L-Asp, L-Thr, and L-Tyr inhibit M. marinum proliferation in zebrafish larvae. (a) Process used to calculate bacterial burden in larvae. Original image (i); image after fluorescence pixelation—red pixels represent relative bacterial burden size (ii); area calculation (iii). (b) Images showing M. marinum burden distribution in wild-type zebrafish larva treated with control, RFP, L-Asp, L-Tyr, and L-Thr (10 μM) for 3 days. White arrows show M. marinum infection areas. (c) Bacterial burden in M. marinum-infected larvae treated with control, RFP (10 μM), or amino acids (10 μM) for 3 days. Individual values are indicated in a scatter dot plot in (c). Student’s t-tests (c) were used. p < 0.05 was statistically significant. Scale bar = 200 μm in (b).