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Figure 2
Compromised number and distribution of HuC/D neurons in MCT8MO embryos at specific stages of development. (A) Representative maximum projection images of the pan-neuronal marker HuC/D immunostaining (white) in the spinal cord between somite 8-12. Comparison of the pattern of neuron distribution in the spinal cord between CTRLMO and MCT8MO embryos at different stages of development. Red highlight - dorsal views, anterior spinal cord up. Blue highlight - lateral view, anterior spinal cord right. Green highlight -transversal view, dorsal spinal cord up. Scale bars represent 25 µm. (B) Quantification of the number of HuC/D single positive cells in a 2-myotome length of the spinal cord. n=9-17. CTRL (CTRLMO); MO (MCT8MO). Results are presented as mean ± SD; Statistical significance determined by t-test: two-sample, assuming equal variances: *p<0.05; ***p<0.001.