ZFIN Image: Dranow et al., 2023, Fig. 4.

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Figure Caption

Fig. 4.

Cell polarity is disrupted in ror2 mutants. (A-F?) Wild type (WT) and ror2^?/? cartilages showing Palatoquadrate cartilages (A-B?), anterior neurocrania (C-D?) and ceratohyal cartilages (E-F?). Panels A?, B?, C?, D?, E? and F? are magnified views of the boxed regions in panels A, B, C, D, E and F, respectively. Arrows point to trabecular cartilages. (G) Quantifications of trabecular cell major axis-to-minor axis. ***P<0.001 (Wilcoxon rank-sum test). Box plot shows the median (middle bar) and first to third interquartile ranges (boxes); whiskers indicate 1.5� the interquartile ranges; dots indicate data points. n=195 WT and 379 ror2^?/? tr cells. (H) Diagram of symplectic cartilage stacking and representative examples of 5 dpf Alcian Blue-stained symplectic cartilages. (I-N) Cell polarity measurements. Representative WT (I,K) and ror2^?/? (J,L) sox10:lyn-tdTomato transgenic craniofacial cartilages stained with anti-?-tubulin antibody in white. Panels I-L? are a single slice of a z-stack. DAPI in blue, anti-?-tubulin in white and sox10:lyn-tdTomato in red for I-L?. Panels K? and L? are magnified views of symplectic cartilages in K and L, respectively. (M) Diagram of how polarity was determined in symplectic cartilages. (N) Quantification of the distribution of the microtubule-organizing center (MTOC) in WT and ror2^?/? symplectic cartilage cells. P<0.001 (Watson's two-sample test for homogeneity). ch, ceratohyal cartilage; ep, ethmoid plate; hm, hyomandibula; Mc, Meckel's cartilage; pq, palatoquadrate cartilage; sy, symplectic cartilage; tr, trabecular cartilage. Scale bars: 100 ?m for A,B,C,D,E,F; 25 ?m for A?,B?,C?,D?,E?,F?; 20 ?m for I-L; 10 ?m for K?,L?.

Acknowledgments

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