ZFIN Image: Vicente et al., 2022, Fig. 4

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Figure Caption

Fig. 4 Tg-plnb^R9C larvae displayed a blunted β-adrenergic response at 3 dpf. A) Ratiometric images from beating hearts (taken at ventricular systole) and their corresponding atrial and ventricular Ca²⁺ traces from representative sibling, Tg-plnb^wt and Tg-plnb^R9C larvae in basal condition and after 30-min incubation in 100 μM isoproterenol. B) Fold change of isoproterenol over basal values of the HR, average ratio (Ca²⁺ level), CaT amplitude, FS, FAC and EF in the ventricle of sibling (n = 24), Tg-plnb^wt (n = 17) and Tg-plnb^R9C (n = 19) larvae. Statistical analysis was performed using a one-way ANOVA test with Tukey's multiple comparisons post-test. Data are shown as mean ± SD (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001). The microscopy chamber temperature was set at 25 °C.

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Reprinted from Journal of Molecular and Cellular Cardiology, 173, Vicente, M., Salgado-Almario, J., Valiente-Gabioud, A.A., Collins, M.M., Vincent, P., Domingo, B., Llopis, J., Early calcium and cardiac contraction defects in a model of phospholamban R9C mutation in zebrafish, 127-140, Copyright (2022) with permission from Elsevier. Full text @ J. Mol. Cell. Cardiol.