Fig. 6

gmfg regulates Yap activity. a Quantitative RT-PCR analysis of klf2a expression in FACS-sorted HE of control and gmfg-atgMO embryos at 48 hpf. Bars represent mean ± SD (n = 3). ns, not significant. b WISH analysis of klf2a expression in the trunk and the cloaca (red arrowheads) of control and gmfg-atgMO embryos at 36 and 48 hpf, respectively. Numbers at the lower right corner of the picture represent embryos with displayed phenotype/whole embryos. Scale bars, 100 µm. c Western blotting showing the protein level of Klf2a in the dissected trunk and tail of control and gmfg-atgMO embryos at 54 hpf. Representative blot is shown in the figure (Full-length blots are presented in Additional file 2: Fig. S4). Data represent mean ± SEM intensity of indicated blots (n = 4). ns, not significant. d Quantitative RT-PCR analysis of yap1, ctgfa and cyr61 expression in FACS-sorted HE of control and gmfg-atgMO embryos at 48 hpf. Bars represent mean ± SD (n = 3). ns, not significant; **p < 0.05, ***p < 0.001. e Western blotting showing the protein level of Yap, Ctgf and p-Yap (S127) in the dissected trunk and tail of control and gmfg-atgMO embryos at 48 hpf. Representative blot is shown in the figure (Full-length blots are presented in Additional file 2: Figs. S5–S7). Data represent mean ± SEM intensity of indicated blots (n = 3). ns, not significant; *p < 0.05. f Western blotting showing the protein level of GMFG, total YAP, CTGF, nuclear and cytoplasmic YAP in HUVEC treated with ctl-sh, gmfg-sh2 and gmfg-sh3. Representative blot is shown in the figure (Full-length blots are presented in Additional file 2: Figs. S8–S12). Data represent mean ± SEM intensity of indicated blots (n ≥ 3). ns, not significant; *p < 0.05; **p < 0.01; ***p < 0.001, ****p < 0.0001