ZFIN Image: Su et al., 2021, Fig. 4

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Figure Caption

Fig. 4

Evaluation of the functional role of Cd40 and Cd154 in TD antigen-induced IgZ immune response. (A, B) Examination of TcpAh-specific IgZ and IgM antibody titers in different tissues and serum samples of zebrafish at 7 and 21 days post-immunization by ELISA. (C) Examination on the percentage of Cd40⁺IgZ⁺ B cells in different tissues by FCM analysis, in which the different treatments were presented at the top of each block diagram. Each diagram is a representative of the three independent experiments. (D) Histogram shows the statistical analysis of the percentage of Cd40⁺IgZ⁺ B cells in each tissue as examined in the FCM analysis. All data were obtained from at least three independent experiments (n = 30). ^⁎p < 0.05, ^⁎⁎p< 0.01, ^⁎⁎⁎p < 0.001.

Acknowledgments

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