Fig. 3

Evaluation on the functional role of Cd40 and Cd154 in vaccinated immunoprotection. (A) Depletion of Cd154⁺ cells or blockade of Cd154 protein impaired the vaccinated immunoprotection of zebrafish against A. hydrophila (1 × 10⁵ CFU/fish) challenge. Kaplan-Meier survival curves represent data pooled from three independent experiments (n = 30). (B) Histogram shows the statistical analysis of the survival rate of fish (n = 30) in different treatment groups after infection with A. hydrophila for 50 h. All data were obtained from at least three independent experiments (n = 30). ^⁎p < 0.05, ^⁎⁎p< 0.01, ^⁎⁎⁎p < 0.001.