Fig. 2
Evaluation on the functional role of Cd40/Cd154 in Cd40+IgZ+ B cell proliferation in immune-related tissues of zebrafish with or without in vivo depletion or blockade of Cd154. (A) Examination on the percentage of Cd40+IgZ+ B cells in different tissues by FCM analysis, in which the different treatments were presented at the top of each block diagram. Each diagram is a representative of the three independent experiments. (B, C) Histogram shows the statistical analysis of the percentage of Cd40+IgZ+ B cells in each tissue as examined in the FCM analysis. All data were obtained from at least three independent experiments (n = 30). ⁎p < 0.05, ⁎⁎p< 0.01, ⁎⁎⁎p < 0.001.