ZFIN Image: Su et al., 2021, Fig. 1

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Figure Caption

Fig. 1

Examination on the expression of cd40/cd154 and igz genes in immune-related tissues and distribution of Cd40 on IgZ⁺ B cells of zebrafish. (A − C) Real-time PCR analysis for the expression of Cd40, Cd154 and igz in spleen, gill, skin, gut and head kidney tissues in A. hydrophila-stimulated groups. All data were obtained from at least three independent experiments (n = 30). ^⁎p < 0.05, ^⁎⁎p< 0.01, ^⁎⁎⁎p < 0.001. (D) Immunofluorescence staining of Cd40 and IgZ on leucocytes. Cells were stained with rabbit anti-Cd40 (green) together with mouse anti-IgZ (red). DAPI (blue) shows the location of the nuclei. The images were obtained using a two-photon laser scanning confocal microscope (Zeiss LSM-710; original magnification, 630×). Scale bars, 30 μm or 10 μm.

Acknowledgments

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