Figure 7
Histologic analysis of hepatic tissue. The zebrafish were injected with carboxymethyllysine (CML) in the presence or absence of CIGB-258 under the consumption of a high-cholesterol diet (HCD). (A) Visualization of the infiltration of neutrophils by Hematoxylin & Eosin (H&E) staining as shown in the left photographs (photographs a, b, c). The right photographs show the conversion of the Hematoxylin-stained area into red intensity (photographs d, e, f). (B) Quantification graph of the nucleus area from H&E staining using Image J software version 1.53r (http://rsb.info.nih.gov/ij/ (accessed on 17 January 2023)) as shown in red intensity. The cell infiltration area was quantified as a percentage of the total hepatocyte area, as determined in H&E-stained sections. The scale bar indicates 100 μm.