Figure 2

Glycation extent of apoA-I in the lipid-free state by carboxymethyllysine (CML) in the presence and absence of CIGB-258. (A) Measurement of the fluorescence intensity (Ex = 370 nm, Em = 440 nm) of apoA-I during 72 h incubation. The data are expressed as mean ± SEM from three independent measurements with duplicate samples. **, p < 0.01; ***, p < 0.001. (B) Electrophoretic patterns of the apoA-I after 72 h incubation (15% SDS-PAGE). Seven μg of protein in apoA-I was equally applied to each lane. Lane 0, apoA-I alone; lane 1, apoA-I (1 mg/mL) + PBS; lane 2, apoA-I + CML (final 200 μM); lane 3, apoA-I + CML + CIGB-258 (final 1 μM); lane 4, apoA-I + CML + CIGB-258 (final 10 μM); lane 5, apoA-I + CML + CIGB-258 (final 100 μM); lane M, molecular weight marker (Bio-Rad, pre-stained low-range).