ZFIN Image: Cho et al., 2023, Figure 1

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Figure Caption

Figure 1

Glycation of HDL₃ (2 mg/mL) by treatment of carboxymethyllysine (CML) and fructose (fruc) at 37 °C in the presence of 5% CO₂. (A) Comparison of fluorescence intensity (Ex = 370 nm, Em = 440 nm) from the glycated product in HDL₃ (200 μg) with the treatment of either CML (final 0, 100, 200, and 400 μM) or fructose (final 0 and 400 μM) during 144 h The data are expressed as mean ± SEM from three independent measurements with duplicate samples. *, p < 0.05; **, p < 0.01; ***, p < 0.001. (B) Electrophoretic patterns (15% SDS-PAGE) of glycated HDL₃ with the treatment of either CML (final 0, 100, 200, and 400 μM) or fructose (final 0 and 400 μM) at 144 h. Five micrograms of total protein in HDL₃ was equally applied to each lane. Red font indicates band intensity from three independent SDS-PAGE analyses. Lane N, native HDL₃; lane M, molecular weight marker (Bio-Rad, prestained low-range).

Acknowledgments

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