ZFIN Image: Yamaguchi et al., 2023, Figure 1

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Figure Caption

Figure 1

Mutation of armc4 causes abnormal motility of Kupffer’s vesicle cilia.

(A) Genomic organization of zebrafish armc4 gene. Black boxes: exons. Gray boxes: untranslated regions. Red asterisk: the genome-editing target site. (B) CRISPR/Cas9 target sequence. (C) Sanger sequencing of armc4^+/+ and armc4^−/− fish around the genome-editing target site. The 8 bp-insertion in armc4^−/− includes a stop codon (red asterisk). (D) Typical kymographs of Kupffer’s vesicle cilia in WT and armc4^−/− embryos. Kymograph patterns were categorized into three classes: rotating (blue), irregular (green), and immotile (red). Scale bar: 100 ms. (E) Ratios of each motility class. Number of cilia: 58 (WT) and 100 (armc4^−/−). (F) Rotational frequencies of Kupffer’s vesicle cilia. Number of cilia: 58 (WT) and 32 (armc4^−/−). Boxes correspond to the first and third quartiles, lines inside the boxes indicate the medians, and whiskers extend to the full range of the data. p-Value was calculated with Welch’s t-test. (G) Directions of heart looping. Number of embryos: 110 (WT) and 63 (armc4^−/−). For comparison, calaxin^-/- data from Sasaki et al., 2019 are displayed in (E, F, and G).

Acknowledgments

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