ZFIN Image: Yamaguchi et al., 2023, Figure 7

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Figure Caption

Figure 7

Cryo-ET structures of OADs in different Ca²⁺ conditions.

(A–B) OAD-DC structures of WT sperm flagella in different Ca²⁺ conditions: (A) 1 mM EGTA condition (for Ca²⁺-free) and (B) 1 mM Ca²⁺ condition. (C) OAD-DC structure of calaxin^-/- sperm flagella incubated with recombinant Calaxin protein in 1 mM Ca²⁺ condition. (A’-C’) Composite images of difference map (red; subtraction of calaxin^-/- in 1 mM EGTA condition from A, B, and C, respectively) and calaxin^-/- OAD-DC (translucent). Black arrowheads in A indicate the CCDC151/114 structure adjacent to Calaxin, which is not observed in B and C. White arrowheads in B and C indicate the additional density around DC, which is not observed in A. (D) OAD-DC structure of calaxin^-/- sperm in 1 mM EGTA condition, which was used to generate (A’-C’).

Acknowledgments

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Figure 7

Figure 7

Cryo-ET structures of OADs in different Ca2+ conditions.

Cryo-ET structures of OADs in different Ca²⁺ conditions.