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Figure 5
Position of zebrafish in the vibrating droplet. (A) Positions of different types of zebrafish indicate that in the droplet with four recirculating flow zones, zebrafish with disrupted mobility (i.e., type II and the ones with lipin-1 deficiency) can be better distinguished from the wide type ones (i.e., type I) as compared to the two recirculating flow conditions. (B–H) Position (i.e., angle) distribution of different types of zebrafish in various flow patterns including two and four recirculating flow patterns. (I) In the four recirculating flow patterns, anesthetized zebrafish gradually regains mobility and moves from 0° to 90° within a time span of 20 min. In contrast, zebrafish with lipin-1 deficiency remain immobile throughout the experiments. In (B), each zebrafish was maintained in the droplet for 20 min at 100% RH and 28.5°C to ensure that the random movement of zebrafish is recorded. Movements of 5–10 zebrafish are individually monitored in droplets with various flow patterns and also more than 50 zebrafish in droplets with no flow.