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Figure 7
BBR induction of autolysosome formation in VECs. A Cell immunofluorescence analysis showing the subcellular co-localization of p62-LC3B, LAMP2-LC3B, LOX-1-LC3B and LOX-1-LAMP2 with their Pearson correlation coefficients and particle numbers (n=10), respectively. The detailed figures of the colocalized particles are shown in Fig. S6A and B. The bars indicate 15 μm. B Autophagosomes and autolysosomes in VECs were observed by transmission electron microscopy, and the particle numbers of autophagosomes and autolysosomes are shown in Fig. S6C (n = 20). Blue boxes indicate representative autophagosomes and red boxes indicate representative autolysosomes or amphisomes, and these structures are amplified below. The BBR concentration was 1 μM, and the oxLDL was 100 μg/mL. The bars indicate 5 μm.