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Figure 6
BBR induction of autolysosome formation in VSMC. A Cell immunofluorescence analysis showing the subcellular co-localization of p62-LC3B, LAMP2-LC3B, LOX-1-LC3B and LOX-1-LAMP2 with their Pearson correlation coefficients and the particle counts (n = 10). The detailed figures of the colocalization particles are shown in Fig. S5A and B. The bars indicate 15 μm. B showing Autophagosomes and autolysosomes in VSMCs under monoculture and co-culture conditions as observed by transmission electron microscopy, and the particle numbers of autophagosomes and autolysosomes are shown in Fig. S5C (n = 20). The BBR concentration was 1 μM, and the oxLDL was 100 μg/mL. Blue boxes indicate representative autophagosomes and red boxes indicate representative autolysosomes or amphisomes, and these structures are enlarged below. The aggregated autophagosomes are shown in the right blue box in the group of co-culture plus oxLDL.