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Figure 5
BBR inhibits EMT and decreases LOX-1 via promotion of autolysosomal digestion in VSMCs and VECs. A, B Western blot analysis shows that the autophagy inhibitor CQ blocked BBR's anti-EMT role in VSMCs (A) and VECs (B). *P < 0.05 vs monoculture ctrl of VSMC (A) or VEC (B), #P < 0.05 vs VSMC (A) or vs VEC (B) under VEC-VSMC co-culture plus oxLDL. C, D Co-immunoprecipitation analysis with anti-p62 and anti-LAMP2 antibodies shows the interaction between the autophagy proteins p62, LC3B, and LAMP2 and LOX1 promoted by BBR in VSMCs (C) and VECs (D) under the condition of VEC-VSMC co-culture plus oxLDL. All western blot data were from independent tests (n = 4 or 5). *P < 0.05 vs monoculture ctrl of VSMCs (C) or VECs (D); #P < 0.05 vs co-culture ctrl of VSMCs (C) or VECs (D).