|
Figure 3
BBR-related inhibition of oxLDL-induced bulge-like proliferation of VSMCs and EMT of VECs. A VSMC migration ability was measured by Transwell assays (scale bar, 200 µm). B Cell immunofluorescence assay confirming the VSMC migration and ectopic expression of VEC and VSMC marker proteins. The antibody against α-SMA protein is labeled with red fluorescence, and the anti-VE-cadherin antibody is labeled with green fluorescence (scale bar, 50 μm). C Western blot results show the levels of VE-cadherin and α-SMA proteins affected by the different treatments. The western blot results from the co-culture condition were semi-quantitatively analyzed, and the data are presented in Fig. S3C (n = 5). The used concentrations were 1 μM for BBR and 100 μg/mL for oxLDL. VEC: human umbilical vein endothelial cells; VSMC: human vascular smooth muscle cells.