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Figure 2
BBR attenuated vascular lipid accumulation and vascular occlusion aggravated by LOX-1 in the HCF-treated zebrafish. A Lipid accumulation in blood vasculature and heart was observed by whole-body ORO staining and Nile Red staining (n = 20). ORO-stained blood vessels are observed at the following body parts: the brainstem (yellow oval ring), the heart (pink oval ring), and the trunk (blue rectangle) (left panel). In the Nile Red staining of the Tg(fli1:GFP) larvae, the green fluorescence represents blood vessels, and the red fluorescence indicates lipid deposition (right panel). The bars indicate 300 μm. B Visible pathological changes of blood vessel structure in zebrafish using HE staining of paraffin sections (left panel). The black arrow points at a part of the blocked vessel. BBR (20 μM) and ATC (1 μM) were respectively given to the HCF plus LOX-1 group. Cholesterol deposition (marked by cholesteryl-BODIPY) in blood vessel walls was observed in transgenic zebrafish Tg(fli1:GFP) using a laser scanning confocal microscope (n = 10) (right panel). The bars indicate 100 μm.