ZFIN Image: Zheng et al., 2023, Figure 1

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Figure Caption

Figure 1

Effects of BBR on the HCF-induced disorders of lipid metabolism in zebrafish larvae. A Attenuation of the HCF-induced lipid accumulation by BBR, as indicated by whole-body ORO staining (n = 20). Colored ovals indicate the following tissues: heart (yellow oval circle), liver (blue oval circle), brain (black oval circle), and trunk vessels (green oval circle). The scale bar represents 200 μm. B Total cholesterol (TC) and total triglycerides (TG) were reduced at the administered concentration of BBR. Samples were collected from 20 zebrafish and tested in triplicate. C Abnormal BMI was corrected by BBR treatment. The BMI was calculated as the ratio of body weight (kg) to body length squared (m²) (n = 10). D BBR-related reduction of high-cholesterol-induced lipid accumulation in liver in both paraffin sections with hematoxylin-eosin staining (HE staining, upper panel) and frozen sections with ORO staining (lower panel) (n = 10). The scale bars represent 15 μm. BBR concentration was 20 μM in (C) and (D). #P < 0.05 vs HCF. BBR: berberine; HCF: high-cholesterol food; and ORO: Oil Red O; ATC: Atorvastatin (positive control).

Acknowledgments

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