ZFIN Image: Liu et al., 2022, Fig. 9.

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Figure Caption

Fig. 9.

cxcl12b is expressed in pharyngeal pouches and is required for PAA morphogenesis. (A) Deletion of cxcl12b led to an interruption of blood flow in the posterior PAAs. Wild-type (WT) embryos and cxcl12b^−/− mutants were injected with rhodamine-dextran into the common cardinal vein at 72 hpf. The blood flow in PAAs was imaged using a Nikon A1R+ confocal microscope. (B) Analysis of tie1 expression in wild-type (WT) embryos and cxcl12b^−/− mutants. (C) Wild-type (WT) and cxcl12b^−/− embryos on Tg(nkx2.5:ZsYellow) background were immunostained using anti-p-AKT (green) and anti-ZsYellow (red) antibodies. (D) Expression analysis of cxcl12b in wild-type embryos during PAA development. Black arrowheads indicate the expression of cxcl12b in the pharynx. (E) Colocalization analysis of cxcl12b transcripts with GFP proteins in pharyngeal pouches. Tg(sox17:GFP) transgenic embryos stained using anti-GFP (green) antibody were hybridized with cxcl12b probe (red). Scale bars: 50 μm.

Acknowledgments

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