ZFIN Image: Liu et al., 2022, Fig. 4.

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Figure Caption

Fig. 4.

AKT is required for stabilization of zEtv2 and zScl. (A,B) Protein levels of zEtv2 and zScl were decreased in PAA clusters of cxcr4a^−/− mutants. (C,D) Expression analysis of zEtv2 and zScl in wild type (WT) and cxcr4a^−/− mutants on the Tg(nkx2.5:ZsYellow) background treated with 10 μM MK-2206 or 0.5 μM SC79 from 18 to 38 hpf. (E-H) HEK293T cells were transfected with indicated plasmids. (E,G) 24 h later, cells were treated with CHX (20 μg/ml) for the indicated times and harvested for immunoblotting. (F,H) Protein levels were quantified and normalized to tubulin (mean±s.d., three independent biological replicates). Scale bars: 50 μm.

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