|
Fig. 2.
Overexpression of ghrelin or deletion of Pax4 potentiates ?-cell regeneration. (A) Experimental scheme for DMSO or MTZ treatment of the wild-type, ghrelin?/? or Tg(hsp70l:ghrl-HA-p2a-mCherry) larvae under the Tg(ins:CFP-NTR; ghrl:Dendra2) background. (B) Overexpression of ghrelin led to an increased number of neogenic CFP+ cells, including Dendra2+ CFP+ double-positive cells (arrowhead) compared with the wild-type MTZ-treated group, but not in the ghrelin?/? mutant. (C) Numbers of CFP+ cells and proportions of Dendra2+ CFP+ double-positive cells per embryo at different stages (n=20). (D) Experimental scheme for DMSO or MTZ treatment of the wild-type, pax4?/? or Tg(hsp70l:pax4-HA-p2a-mCherry) larvae under the Tg(ins:CFP-NTR) background. (E) The numbers of neogenic CFP+ cells were significantly increased in the pax4?/? mutant, and overexpression of pax4 could rescue the increased ?-cell regeneration in the pax4?/? mutant. (F) The numbers of CFP+ cells per embryo at different stages (n=20). All statistical data are expressed as meanħs.e.m. P-values were calculated using an unpaired Student's t-test. Scale bars: 10 µm.