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Figure 8
BBR suppresses LOX-1 and EMT by activating autophagy in zebrafish. Whole-mount immunofluorescence experiments were performed to detect co-localization between LOX-1 protein and autophagolysosomal proteins (LC3B and LAMP2). A, B Co-localized particles of LC3B with LOX-1 (A) and LAMP2 with LOX-1 (B) were observed in the HCF plus BBR and the HCF plus BBR plus CQ groups. The results were supported by the Pearson correlation coefficient and counts of the co-localized particles. Respective particle counts of LOX-1, LC3B and LAMP2 showed that LOX-1 protein particles was decreased by BBR treatment, but CQ suppressed the BBR effect compared to the HCF group (n=10). The bars indicate 50 μm. *P < 0.05 vs ctrl, #P < 0.05 vs HCF. The detailed images of LOX-1 co-localization with LC3B and with LAMP2 are shown in Fig. S7 . C Western blot showing LOX-1 protein levels in whole zebrafish in the different treatments. D showing BBR effects on LOX-1 level and autophagy flux in a concentration-dependent manner. E Levels of EMT markers, LOX-1 and autolysosome markers in the different groups in vivo. The data in C-E were from 15-dpf larvae (20 zebrafish) (n = 5). *P < 0.05 vs ctrl, #P < 0.05 vs HCF.