Figure 1
Pg induced a reduction in the tumor xenograft area of ACC cells. (A) Representative, lateral-view pictures of Tg (kdrl: EGFP) control and Pg-treated embryos at 120 hpf. Each ACC cell line was labeled with a red fluorescent lipophilic dye while the embryo endothelium was labeled with a green fluorescent protein reporter driven by the kdrl promoter. Images were acquired using a Zeiss LSM 510 META confocal laser scanning microscope at 10× magnification. (B) Tumor areas at 48 hpf (T0—the start of treatment) and 120 hpf (T3—end of treatment) of drug-treated and vehicle-treated groups measured using Zen 2.3 Black software from ZEISS. Data are shown as the mean of independent experiments ± SEM. * p < 0.0001; § p < 0.05.