Fig. 5

Fig. 5

Fig. 5. 15-keto-PGE₂ exposure affects podocyte intercalation process during glomerular vascularization. (a) Representative confocal microscopy images of Tg[wt1b:eGFP] zebrafish glomeruli at 48 hpf after DMSO vehicle 0.88 % and 15-keto-PGE₂ 500 μM treatment; N = 2, n = 5 for both conditions; dotted-line circle indicates podocyte consolidation and failure of normal elaboration around the continuously forming glomerular capillaries. (b) Quantification graph showing the percentage of imaged glomeruli with affected morphology at 48 hpf after exposure to DMSO (not affected morphology) and 15-keto-PGE₂ (4 out of 5 imaged glomeruli showed affected morphology). (c) Representative confocal microscopy images of Tg[wt1b:eGFP] zebrafish glomeruli at 96 hpf after DMSO vehicle 0.88 % and 15-keto-PGE₂ 500 μM treatment; N = 3, n = 7 for DMSO and N = 3, n = 6 for 15-keto-PGE₂; dotted-line circle indicates impaired podocyte intercalation around the formed capillaries. (d) Quantification graph showing the percentage of imaged glomeruli with affected morphology at 96 hpf after exposure to DMSO (not affected morphology) and 15-keto-PGE₂ (5 out of 6 imaged glomeruli showed affected morphology). In all images, grey scale images show the podocytes and parietal epithelial cells in the glomeruli; in the merge podocytes and parietal epithelial cells are represented in green (eGFP), endothelial cells of glomeruli capillaries are marked in magenta (BSA-AlexaFluor555) and cells nuclei in blue (DAPI); Scale bar = 10 μm. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)