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Figure 5
LC3 and LAMP1 colocalization and BODIPY-SM distribution were impaired following Hh and HDAC6 inhibition. (A) Double immunostaining against LC3 and LAMP1 of U87-MG cells treated in the presence or absence of both 8 ?M of TubA and 10 ?M of cyclo. Asterisks show some LC3 puncta, indicating autophagosomes, that did not colocalize with the red ones, indicating lysosomes. (B) BODIPY-SM and LysoTracker Red DND-99 staining of U87-MG cells treated in the presence or absence of both 8 ?M of TubA and 10 ?M of cyclo. CT?control, TubA/T?tubastatin A; cyclo/C?cyclopamine. Scale bar indicates 40 ?m.
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