ZFIN Image: Watchon et al., 2023, Figure 3

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Figure Caption

Figure 3

Adult SCA3 zebrafish do not exhibit impairment in the autophagy pathway. (A) Representative Western blot of 7-month-old SCA3 zebrafish brain and spinal cord protein samples. Western blot was probed with beclin-1, p62, and LC3B. (B) Quantification of beclin-1 revealed no significant differences between the genotypes (n = 4–6). (C) Quantification of p62 revealed a decrease in the ataxin-3 84Q zebrafish compared to the ataxin-3 23Q zebrafish and the non-transgenic control (p = 0.0106 and p = 0.0254, respectively, n = 7–9). (D) Quantification of LC3II/LC3I ratio showed no significant differences between the genotypes (n = 6–9). NT-Non-transgenic, FL-full-length, ZF-zebrafish. Error bars represent mean ± SEM. Data points represent individual adult zebrafish Statistical analysis performed was a one-way ANOVA followed by a Tukey post hoc analysis. * represents p < 0.05.

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