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Fig. 5
A: Success rates of natural mating of WT♂×WT♀, WT♂×(-/-)♀, WT♀×(-/-)♂, and (-/-)♂×(-/-)♀ were detected ( n=10 pairs of fish for each group). B: Contact frequencies between WT♂×WT♀, WT♂×(-/-)♀, WT♀×(-/-)♂, and (-/-)♂×(-/-)♀were detected ( n=3 pairs of fish for each group). C: Fertilization rate calculated from in vitro fertilization using WT sperm+WT egg (WT in-cross), scd -/- egg ([-/-]♀)+WT sperm (WT♂), scd -/- sperm ([-/-]♂)+WT egg (WT♀), and scd -/- sperm ([-/-]♂)+ scd -/- egg ([-/-]♀) ( n=3 pair groups were analyzed for each). D: Four natural fertilization groups. E: Representative images from in vitro fertilization of scd -/- sperm ([-/-]♂)+ scd -/- egg ([-/-]♀) showing natural fertilized egg development. F: Survival rate of scd -/- mutant larvae was ~30%, significantly lower than that of WT larvae. G, H: Morphological analysis of secondary sexual characteristics. WT females had a rounded body shape, silver and black body color, white anal fin (g2), and large extended genital papilla (g1). However, scd -/- females had shorter and rounded body shape, silver and black body color, white anal fin (g4), and extended genital papilla (g3). The scd -/- /Tg(CMV:scd) and WT females had the same secondary sexual characteristics (g5, g6). WT males had slim body shape, orange and black body color, orange and black anal fin (h2), and small genital papilla (h1). However, scd -/- males had shorter and curved body shape, orange and black body color, orange and black anal fin (h4), and no genital papilla (h3). The scd -/- /Tg(CMV:scd) and WT males had the same secondary sexual characteristics (h5, h6). All values are mean± SEM. Student t-tests were used. **: P<0.01; ***: P<0.001. N/A: Not available.