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Fig. 3
Fig. 3. Silica crystals promotes local and systemic inflammation in zebrafish larvae. SiO2 crystals or PBS were injected in the hindbrain ventricle (HBV, dash line) of 2 dpf Tg(NFkB-RE:eGFP) (A-D) and Tg(il1b:eGFP) (E-H). Nfkb activation (A-D) and Il1b production (E-H) were analyzed by fluorescence microscopy from 1 to 48 hpi (A, B, E, F) or 24 hpi (C, D, G, H). Each dot represents one individual and the mean ± S.E.M. for each group is also shown. P values were calculated using one-way ANOVA and Tukey multiple range test. ns, not significant, *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001. Bars: 100 μm (A, E), 500 μm (C, G).