Figure 5
TSE ameliorated ethanol-induced metabolism dysregulation in vivo. (A) qRT-PCR analysis of the mRNA levels of cyp2y3, cyp3a65, adh8a, and adh8b in zebrafish, n = 3 biologically independent samples. (B) qRT-PCR analysis of the mRNA levels of Cyp3a4, Cyp3a7, Cyp2d6, and Cyp2c45 in liver tissue, n = 3 biologically independent samples. Activity of (C) CYP2E1, (D) ALDH, and (E) ADH in the chicken embryo liver tissues, n = 6 biologically independent samples. Data represent the mean ± standard deviation and significant differences were analyzed by one-way ANOVA. # p < 0.05, ## p < 0.01, ### p < 0.001 vs. control group; * p < 0.05, ** p < 0.01, *** p < 0.001 vs. ethanol group; ns, not significant vs. ethanol group.