ZFIN Image: Han et al., 2023, Figure 2

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Figure 2

TSE protected HepG2 cells against ethanol-induced damage. (A) MTT assay of HepG2 cell viability following treatment with TSE for 24 h, n = 4 biologically independent experiments. (B) MTT assay of HepG2 cell viability following treatment with ethanol for 24 h, n = 4 biologically independent experiments. (C) MTT assay of HepG2 cell viability following pretreatment with or without TSE for 24 h before cotreatment with or without ethanol (700 mM) for 3 h, n = 4 biologically independent experiments. (D) Morphology of cells under microscope (magnification �200). (E) Flow cytometric analysis of intracellular ROS levels detected by DCFH-DA probe, n = 4 biologically independent experiments. (F) Flow cytometric analysis of mitochondrial membrane potential by rhodamine 123 staining, n = 4 biologically independent experiments. Data represent the mean � standard deviation and significant differences were analyzed by one-way ANOVA. ^### p < 0.001 vs. control group; *** p < 0.001 vs. ethanol group. ns, not significant vs. ethanol group.

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