FIGURE 2

The EWSR1 knockdown promotes induction of lagging chromosome during anaphase. (A). Schematic diagram for the mitotic synchronization of EWSR1 knockdown cells using Thymidine and Nocodazole, followed by the mitotic shake-off and release from the mitotic arrest for 60 min. (B). Representative images of chromosomes; normal (top panel), lagging chromosome (middle panel) and chromosome bridge (bottom panel). Yellow square highlights the magnified images of either lagging chromosome or chromosome bridge. Chr.; chromosome, Scale bar = 10 um. (C). Percentages of anaphase cells with lagging chromosomes (left graph) and of chromosomal bridges (right graph) in AUX- and AUX + cells (Total 34-104 anaphase cells per sample, n = 3 experiments). *p < 0.05. (paired t-test), NS; Non-Significant.